Automated segmentation and morphometry of cell and tissue. Automated segmentation and morphometry of cell and tissue structures. Practical applications of morphometric analysis in toxicology. This site is dedicated to helping researchers understand the principles of designbased stereology and its advantages over less sophisticated approaches in quantitative histology. The segmental morphometric properties of the horse cervical. The statistical and mathematical principles used in stereology guarantee that no assumptionsor biasare introduced in the analysis. Most of stereological software is just a part of multifunctional software packages and, therefore, could incur very significant expenses that can interfere with the widespread use of computerized stereological tools. The sections were evaluated using cavalieris method and volume fraction approach. In despite of the argument about nomenclature morphometry and stereology could denominate the same method aherne and dunnill 1982 most authors consider morphometry a twodimensional quantitative method that uses a caliper mainly a caliper micrometer, a gauge with a calibrated micrometer screw for the measurement of thin objects under microscopic observation.
One of the wellknown exceptions is imagej software 1, which is a public domain java image. Enter your mobile number or email address below and well send you a link to download the free kindle app. In vivo stereological assessment of human cerebellar volume. The opportunities of confocal microscopy applied to morphometry of microscopical structures are presented and demonstrated on stereological methods based on evaluation of optical sections within a. The aim of this study was to compare the accuracy of digital image analysis tools and the. Final versions of each program as well as full support and windows compatibility will be available soon, along with the relevant publications. Morphometric applications in anatomic pathology morphometric applications in anatomic pathology true, lawrence d 19960501 00. An official research policy statement of the american.
Tips for studies with quantitative morphology morphometry. Objective to assess the relationship between in vivo measurements of optic disc topography and histomorphometric measurements of optic nerve fiber number in glaucoma. This has caused the method of construction to be rethought as well as methods of restoration. A computed tomography algorithm effectively reconstructs. Software currently in active development the following software is in betatesting phase and as such it may still contain bugs relevant for proper program function.
Results for stereological parameters of the norway spruce needles and their mesophyll anatomical structure obtained using this studys novel stereological methods are shown in table 1. This term is widely used in the stereological literature, which we have made an effort to respect in order to support a standardized terminology. This barcode number lets you verify that youre getting exactly the right version or edition of a book. Although the cervical spinal cord csc of the horse has particular importance in diseases of cns, there is very little information about its segmental morphometry. Aug 27, 2009 by means of the cast software visiopharm, denmark, the stereological probes points, lines, and counting frames were superimposed onto the video images of the tissue sections viewed on the monitor. Morphometric analyses are commonly performed on organisms, and are useful in analyzing their fossil record, the impact of mutations on shape. The set of stereological morphometric grids macros for imagej. Methods both eyes of 10 monkeys macaca fascicularis with laserinduced glaucoma in the right eye were studied. Whereas a polished rock surface is really a 2dobject, tissue sections will be 3d by their translucency. Then you can start reading kindle books on your smartphone, tablet, or computer no kindle device required. We have developed a method to quantitatively analyze the whole. Gene, cell and tissue basic application of stereology in.
When correctly implemented, the sensitivity and efficiency of stereological methods ensures accurate estimates with a fixed and predetermined precision. Morphometrics from greek morphe, shape, form, and metria, measurement or morphometry refers to the quantitative analysis of form, a concept that encompasses size and shape. Despite already available digital methods, the pathologists visual score is still widely considered as ground truth and used as a primary method in histomorphometric evaluations. Sep 23, 2016 the large size of human tissues requires a practical stereological approach to perform a comprehensive analysis of the whole organ. The objective of the present study was to determine the morphometric features of the csc segments in the horse and possible relationships among the morphometric features.
Calibration of the stereological estimation of the number of. Stereological procedures which allow the assessment of volume percentages, surfaces, lengths, numbers, and diameters are discussed in the light of dimension reduction. Biological sciences chemical weapons analysis design and construction. They are recommended reading prior to embarking upon a project requiring morphometric or stereologic analysis methods. Actually, they were first devised as a method of analyzing the mineral composition of various rocks.
The application of stereological methods for estimating. Stereological studies demonstrated that the sand was obtained from dunes facing the bays. Changes induced by physical activity and weight loss in the. In response to the intervention, there were substantial reductions in weight, bmi, and fat mass. We will consider how computers are being used to solve several problems of data management and to identify software packages that currently offer new and improved strategies for biological stereology. Numerous stereological methods have been developed and published, each being specific for different histological tasks. The application of stereological methods to biological studies permits researchers to effectively and efficiently gather unbiased, accurate data. The text by misell 1978 was written prior to the currently available software, but deals extensively with the principles and methods for image enhancement and analysis not confined to computers. All estimates were validated against exhaustive counts of the same nerve. Shtereom i simple windowsr based software for stereology. Application of morphometric and stereological techniques on. Feb 15, 2014 read alterations of mouse lung tissue dimensions during processing for morphometry. Relationship of optic disc topography to optic nerve fiber. In the present report, we apply this stereological method to evaluate the effects of age and gender on human cerebellar volume.
Computer programs for biological stereology springerlink. The comparison of selected mesophyll parameters was conducted to evaluate the effect of simulated acid rain on current norway spruce needles. The use of the letter q to refer to the intercepts of linear features with an area probe originates from the german term querschnitt originally used by weibel in his pioneering work involving the application of stereological methods to biological tissue weibel 1979. Methods defined in this technique are essential for proper understanding of the structure of tissue, cells, cellular organelles, and other particles in the body of living organisms. Quantification of myocardial fibrosis by digital image. Both methods were used in a modified way to correct for variations in section thickness fractionator and to reduce the length of the measuring process weibelgomez, respectively. The optical fractionator technique to estimate cell numbers. Mar 01, 2007 stereology has been earlier defined by wiebel 1970 to be. Software for morphometry images of biological drugs a powerful universal tool, which allows directly to control the process of inputting images, scanning products, as well as methods to automate routine image analysis of biological products and samples. Stereology some systems have been announced to do automatic stereology like c.
Using these methods, differences can be demonstrated which are missed with the routine subjective methods. Shtereom i is a simple windowsbased software for stereological estimation. Stereological techniques are not limited to the biological sciences. Everyday low prices and free delivery on eligible orders. Stereological methods have evolved from being cumbersome and timeconsuming to being efficient and practical because of combined advances in stereological theory, sampling techniques, software, and imaging. Alterations of mouse lung tissue dimensions during. Cardiac fibrosis disrupts the normal myocardial structure and has a direct impact on heart function and survival. Section 1 gives an overview of stereology, sections 2 point counting programs and 3 digitizing programs describe software currently being used for collecting raw data and estimating simple stereological parameters, and section 4 includes a discussion of several specialpurpose programs that offer access to some of the more advanced methods. Stereology the definitive source for stereology information. Here, we present a stereological method, the optical fractionator, used to quantify the formation of new neurons, and their survival, in the rat hippocampus following electroconvulsive stimulation. Morphometric applications in anatomic pathology, human.
The incorporation of data archiving permits easier access to previous studies, as well as the sharing of stereological findings and their related maps throughout the scientific community. Characterization of these quantitative changes provides data that is useful not only for making a. Some of the stereological systems now integrate spatial mapping with stereological analyses to provide more comprehensive methods to analyze brain tissue. Stereology is a powerful, efficient, and reliable set of methods for analyzing the morphology of the lung quantitatively ochs, 2006. Using these two stereological techniques, the mean glomerular volume and the total glomerular number per kidney were determined in j 6 kidneys of 8 rats. Practical methods for biological morphometry vol 1 by weibel, ewald r. Unbiased stereological methods were used to evaluate the adrenal gland morphology. There were significant improvements in aerobic fitness vo 2 max, insulin sensitivity, and fat oxidation after an overnight fast, as reflected in a decrease in fasting rq. Application of morphometric and stereological techniques on analysis and modelling of the avian lung, new insights into morphometry studies, pere m. The segmented csc from five mature animals was used. Much of their practical application today is in the field of metallurgy and modifications of the techniques are used in ecology, geography, and earth sciences.
Mar 15, 2010 besides biological variation, parameters tested included two variations of stereological methods unbiased counting frame versus 2ddisector, two sampling schemes few large versus frequent small sampling boxes, and workstations with varying degrees of sophistication. Stereological methods have been adapted to biological samples and are comprehensively described by weibel. Novel efficient methods for measuring mesophyll anatomical. In despite of the argument about nomenclature morphometry and stereology could denominate the same method aherne and dunnill 1982 most authors consider morphometry a twodimensional. Is length an appropriate estimator to characterize pulmonary. A comparison of methods, ajp lung cellular and molecular physiology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Comparison of two stereological methods for quantitative. Stereology is a completely different enterprise from computed tomography. The large size of human tissues requires a practical stereological approach to perform a comprehensive analysis of the whole organ.